• Western blot analysis of lysates from 293 cells, using NFAT3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using NFAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NFAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using NFATc4 Polyclonal Antibody diluted at 1:500

Anti-NFATC4 antibody (642-691) (STJ94450)

SKU:
STJ94450

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nuclear factor of activated T-cells, cytoplasmic 4 (642-691) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFATC4
Gene ID: 4776
Uniprot ID: NFAC4_HUMAN
Immunogen Region: 642-691
Specificity: NFATc4 Polyclonal Antibody detects endogenous levels of NFATc4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NFAT3. AA range:642-691
Tissue Specificity Widely expressed, with high levels in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. In the hippocampus, expressed in the granular layer of the dentate gyrus, in the pyramidal neurons of CA3 region, and in the hippocampal fissure. Expressed in the heart (at protein level).
Post Translational Modifications Phosphorylated by NFATC-kinases.dephosphorylated by calcineurin/PPP3CA. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7/ERK5 and MAPK14/p38, on Ser-213 and Ser-217 by MAPK8/JNK1 and MAPK9/JNK2, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B. Phosphorylation by GSK3B markedly increases NFATC4 ubiquitination. Phosphorylation at Ser-168 and Ser-170 is stimulated by UV irradiation. Phosphorylation determines subcellular location: the hyperphosphorylated protein is cytosolic, while the dephosphorylated form is targeted to the nucleus. Ubiquitinated, leading to degradation by the proteasome. Ubiquitination may be stimulated by GSK3B-dependent phosphorylation. Polyubiquitin linkage mainly occurs through 'Lys-48'.
Function Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4. Along with NFATC3, involved in embryonic heart development. Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function. Transactivates many genes involved in the cardiovascular system, including AGTR2, NPPB/BNP (in synergy with GATA4), NPPA/ANP/ANF and MYH7/beta-MHC. Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation. In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during the developmental critical period, when auditory neurons depend on afferent input for survival. Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP). Plays a role in adipocyte differentiation. May be involved in myoblast differentiation into myotubes. Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription. Binds to PPARG gene promoter and regulates its activity. Binds to PPARG and REG3G gene promoters.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 4
Nf-Atc4
Nfatc4
T-Cell Transcription Factor Nfat3
Nf-At3
Cellular Localisation Cytoplasm
Cytosol
Nucleus
When Hyperphosphorylated
Localizes In The Cytosol
When Intracellular Ca(2+) Levels Increase
Dephosphorylation By Calcineurin/Ppp3ca Leads To Translocation Into The Nucleus
Mapk7/Erk5 And Mtor Regulate Nfatc4 Nuclear Export Through Phosphorylation At Ser-168 And Ser-170
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 4 antibody
Anti-Nf-Atc4 antibody
Anti-Nfatc4 antibody
Anti-T-Cell Transcription Factor Nfat3 antibody
Anti-Nf-At3 antibody
Anti-NFATC4 antibody
Anti-NFAT3 antibody

Information sourced from Uniprot.org

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