• Immunofluorescence analysis of rat spleen using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Jurkat cells, using (STJ24752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NFATC2 antibody (1-300) (STJ24752)

SKU:
STJ24752

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFATC2 (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFATC2
Gene ID: 4773
Uniprot ID: NFAC2_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NFATC2 (NP_775114.1).
Immunogen Sequence: MNAPERQPQPDGGDAPGHEP GGSPQDELDFSILFDYEYLN PNEEEPNAHKVASPPSGPAY PDDVLDYGLKPYSPLASLSG EPPGRFGEPDRVGPQKFLSA AKPAGASGLSPRIEITPSHE LIQAVGPLRMRDAGLLVEQP PLAGVAASPRFTLPVPGFEG YREPLCLSPASSGSSASFIS DTFSPYTSPCVSPNNGGPDD LCPQFQNIPAHYSPRTSPIM SPRTSLAEDSCLGRHSPVP
Tissue Specificity Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.
Post Translational Modifications In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated.which is required for full transcriptional activity. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
Function Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. Is involved in the negative regulation of chondrogenesis.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 2
Nf-Atc2
Nfatc2
Nfat Pre-Existing Subunit
Nf-Atp
T-Cell Transcription Factor Nfat1
Database Links Reactome: R-HSA-2025928
Reactome: R-HSA-2871809
Reactome: R-HSA-5607763
Reactome: R-HSA-8877330
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated Dephosphorylation
Rapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium Signals
The Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 2 antibody
Anti-Nf-Atc2 antibody
Anti-Nfatc2 antibody
Anti-Nfat Pre-Existing Subunit antibody
Anti-Nf-Atp antibody
Anti-T-Cell Transcription Factor Nfat1 antibody
Anti-NFATC2 antibody
Anti-NFAT1 antibody
Anti-NFATP antibody

Information sourced from Uniprot.org

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