Anti-Nephrin antibody (STJ11108292)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit polyclonal Nephrin antibody for use in WB, IF-F, IF-P, IHC-P and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF-F/IF-P/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1500-1:3000IF-F, 1:50-1:200IF-P, 1:50-1:200IHC-P, 2000-8000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Nphs1 |
| Gene ID | 54631 |
| Uniprot ID | NPHN_MOUSE |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Immunogen Sequence | Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated at Tyr-1208 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-MMU-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocated At Podocyte Slit Diaphragm Between Podocyte Foot Processes |
| Alternative Antibody Names | Anti-Nephrin antibodyAnti-Renal Glomerulus-Specific Cell Adhesion Receptor antibodyAnti-Nphs1 antibodyAnti-Nphn antibody |
Information sourced from Uniprot.org