• Immunofluorescence analysis of NIH/3T3 cells using NEK8 Rabbit polyclonal antibody (STJ24736) at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using NEK8 Rabbit polyclonal antibody (STJ24736) at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NEK8 Rabbit polyclonal antibody (STJ24736) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.

Anti-NEK8 antibody (270-390) (STJ24736)

SKU:
STJ24736

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEK8 (270-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK8
Gene ID: 284086
Uniprot ID: NEK8_HUMAN
Immunogen Region: 270-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-390 of human NEK8 (NP_835464.1).
Immunogen Sequence: GSVRMRRAEKSVAPSNTGSR TTSVRCRGIPRGPVRPAIPP PLSSVYAWGGGLGTPLRLPM LNTEVVQVAAGRTQKAGVTR SGRLILWEAPPLGAGGGSLL PGAVEQPQPQFISRFLEGQS G
Tissue Specificity Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma.
Function Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
Protein Name Serine/Threonine-Protein Kinase Nek8
Never In Mitosis A-Related Kinase 8
Nima-Related Protein Kinase 8
Nima-Related Protein Kinase 12a
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Cilium
Microtubule Organizing Center
Centrosome
Predominantly Cytoplasmic
Localizes To The Proximal Region Of The Primary Cilium And Is Not Observed In Dividing Cells
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek8 antibody
Anti-Never In Mitosis A-Related Kinase 8 antibody
Anti-Nima-Related Protein Kinase 8 antibody
Anti-Nima-Related Protein Kinase 12a antibody
Anti-NEK8 antibody
Anti-JCK antibody
Anti-NEK12A antibody

Information sourced from Uniprot.org

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