Anti-NEK8 antibody (270-390) (STJ24736)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NEK8 (270-390) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IF/ICC:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NEK8 |
| Gene ID | 284086 |
| Uniprot ID | NEK8_HUMAN |
| Immunogen Region | 270-390 |
| Immunogen Sequence | GSVRMRRAEKSVAPSNTGSR TTSVRCRGIPRGPVRPAIPP PLSSVYAWGGGLGTPLRLPM LNTEVVQVAAGRTQKAGVTR SGRLILWEAPPLGAGGGSLL PGAVEQPQPQFISRFLEGQS G |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 270-390 of human NEK8 (NP_835464.1). |
Additional Info
| Tissue Specificity | Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma. |
| Function | Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis, and is involved in the regulation of the Hippo signaling pathway. |
| Protein Name | Serine/Threonine-Protein Kinase Nek8Never In Mitosis A-Related Kinase 8Nima-Related Protein Kinase 8Nima-Related Protein Kinase 12a |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionCiliumMicrotubule Organizing CenterCentrosomeCilium AxonemePredominantly CytoplasmicLocalizes To The Proximal Region Of The Primary Cilium And Is Not Observed In Dividing Cells |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek8 antibodyAnti-Never In Mitosis A-Related Kinase 8 antibodyAnti-Nima-Related Protein Kinase 8 antibodyAnti-Nima-Related Protein Kinase 12a antibodyAnti-NEK8 antibodyAnti-JCK antibodyAnti-NEK12A antibody |
Information sourced from Uniprot.org