Anti-NEK6 antibody (200-300) [S4MR] (STJ11101894)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal NEK6 (200-300) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NEK6 |
| Gene ID | 10783 |
| Uniprot ID | NEK6_HUMAN |
| Immunogen Region | 200-300 |
| Immunogen Sequence | ETTAAHSLVGTPYYMSPERI HENGYNFKSDIWSLGCLLYE MAALQSPFYGDKMNLFSLCQ KIEQCDYPPLPGEHYSEKLR ELVSMCICPDPHQRPDIGYV H |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human NEK6 (Q9HC98). |
Additional Info
| Tissue Specificity | Ubiquitous, with highest expression in heart and skeletal muscle. |
| Post Translational Modifications | Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210. |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. |
| Protein Name | Serine/Threonine-Protein Kinase Nek6Never In Mitosis A-Related Kinase 6Nima-Related Protein Kinase 6Protein Kinase Sid6-1512 |
| Database Links | Reactome: R-HSA-2980767Reactome: R-HSA-3301854Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleColocalizes With Apbb1 At The Nuclear SpecklesColocalizes With Pin1 In The NucleusColocalizes With Atf4Cir1Arhgap33Ankra2Cdc42Nek9Rad26lRbbp6Rps7Trip4Relb And Phf1 In The CentrosomeLocalizes To Spindle Microtubules In Metaphase And Anaphase And To The Midbody During Cytokinesis |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek6 antibodyAnti-Never In Mitosis A-Related Kinase 6 antibodyAnti-Nima-Related Protein Kinase 6 antibodyAnti-Protein Kinase Sid6-1512 antibodyAnti-NEK6 antibody |
Information sourced from Uniprot.org