• Western blot analysis of extracts of various cell lines, using NEK3 antibody (STJ28748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NEK3 antibody (267-506) (STJ28748)

SKU:
STJ28748

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEK3 (267-506) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK3
Gene ID: 4752
Uniprot ID: NEK3_HUMAN
Immunogen Region: 267-506
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 267-506 of human NEK3 (NP_689933.1).
Immunogen Sequence: PEIIMEYGEEVLEEIKNSKH NTPRKKTNPSRIRIALGNEA STVQEEEQDRKGSHTDLESI NENLVESALRRVNREEKGNK SVHLRKASSPNLHRRQWEKN VPNTALTALENASILTSSLT AEDDRGGSVIKYSKNTTRKQ WLKETPDTLLNILKNADLSL AFQTYTIYRPGSEGFLKGPL SEETEASDSVDGGHDSVILD PERLEPGLDEEDTDFEEEDD NPDWVSELKKRAGWQGLCD
Tissue Specificity Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.
Post Translational Modifications Phosphorylation at Thr-479 regulates its catalytic activity.
Function Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.
Protein Name Serine/Threonine-Protein Kinase Nek3
Hspk 36
Never In Mitosis A-Related Kinase 3
Nima-Related Protein Kinase 3
Cellular Localisation Cytoplasm
Cell Projection
Axon
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek3 antibody
Anti-Hspk 36 antibody
Anti-Never In Mitosis A-Related Kinase 3 antibody
Anti-Nima-Related Protein Kinase 3 antibody
Anti-NEK3 antibody

Information sourced from Uniprot.org

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