• Immunofluorescence analysis of SH-SY5Y cells using Neurofilament M Rabbit polyclonal antibody (STJ118833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Neurofilament M in paraffin-embedded mouse brain using Neurofilament M Rabbit polyclonal antibody (STJ118833) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Neurofilament M in paraffin-embedded rat brain using Neurofilament M Rabbit polyclonal antibody (STJ118833) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from SH-SY5Y cells, using Neurofilament M Rabbit polyclonal antibody (STJ118833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NEFM antibody (100-260) (STJ118833)

SKU:
STJ118833

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEFM (100-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFM
Gene ID: 4741
Uniprot ID: NFM_HUMAN
Immunogen Region: 100-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human Neurofilament M (NP_005373.2).
Immunogen Sequence: NEKEQLQGLNDRFAGYIEKV HYLEQQNKEIEAEIQALRQK QASHAQLGDAYDQEIRELRA TLEMVNHEKAQVQLDSDHLE EDIHRLKERFEEEARLRDDT EAAIRALRKDIEEASLVKVE LDKKVQSLQDEVAFLRSNHE EEVADLLAQIQASHITVERK D
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Medium Polypeptide
Nf-M
160 Kda Neurofilament Protein
Neurofilament 3
Neurofilament Triplet M Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Medium Polypeptide antibody
Anti-Nf-M antibody
Anti-160 Kda Neurofilament Protein antibody
Anti-Neurofilament 3 antibody
Anti-Neurofilament Triplet M Protein antibody
Anti-NEFM antibody
Anti-NEF3 antibody
Anti-NFM antibody

Information sourced from Uniprot.org

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