• Immunofluorescence analysis of L929 cells using NEDD1 antibody (STJ24728) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NEDD1 antibody (STJ24728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NEDD1 antibody (411-660) (STJ24728)

SKU:
STJ24728

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEDD1 (411-660) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEDD1
Gene ID: 121441
Uniprot ID: NEDD1_HUMAN
Immunogen Region: 411-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human NEDD1 (NP_690869.1).
Immunogen Sequence: SPIRDDAVVNKGSDESIGKG DGFDFLPQLNSVFPPRKNPV TSSTSVLHSSPLNVFMGSPG KEENENRDLTAESKKIYMGK QESKDSFKQLAKLVTSGAES GNLNTSPSSNQTRNSEKFEK PENEIEAQLICEPPINGSST PNPKIASSVTAGVASSLSEK IADSIGNNRQNAPLTSIQIR FIQNMIQETLDDFREACHRD IVNLQVEMIKQFHMQLNEMH SLLERYSVNEGLVAEIERL
Post Translational Modifications During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation.
Function Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
Protein Name Protein Nedd1
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1
Nedd-1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-Protein Nedd1 antibody
Anti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1 antibody
Anti-Nedd-1 antibody
Anti-NEDD1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance