| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NEDD1 |
| Gene ID: | 121441 |
| Uniprot ID: | NEDD1_HUMAN |
| Immunogen Region: | 411-660 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human NEDD1 (NP_690869.1). |
| Immunogen Sequence: | SPIRDDAVVNKGSDESIGKG DGFDFLPQLNSVFPPRKNPV TSSTSVLHSSPLNVFMGSPG KEENENRDLTAESKKIYMGK QESKDSFKQLAKLVTSGAES GNLNTSPSSNQTRNSEKFEK PENEIEAQLICEPPINGSST PNPKIASSVTAGVASSLSEK IADSIGNNRQNAPLTSIQIR FIQNMIQETLDDFREACHRD IVNLQVEMIKQFHMQLNEMH SLLERYSVNEGLVAEIERL |
| Post Translational Modifications | During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. |
| Function | Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. |
| Protein Name | Protein Nedd1Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1Nedd-1 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosome |
| Alternative Antibody Names | Anti-Protein Nedd1 antibodyAnti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1 antibodyAnti-Nedd-1 antibodyAnti-NEDD1 antibody |
Information sourced from Uniprot.org
