| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S3MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NDUFS4 |
| Gene ID: | 4724 |
| Uniprot ID: | NDUS4_HUMAN |
| Immunogen Region: | 1-100 |
| Immunogen Sequence: | MAAVSMSVVLRQTLWRRRAV AVAALSVSRVPTRSLRTSTW RLAQDQTQDTQLITVDEKLD ITTLTGVPEEHIKTRKVRIF VPARNNMQSGVNNTKKWKME |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NDUFS4 (O43181). |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - MitochondrialComplex I-18 KdaCi-18 KdaComplex I-AqdqCi-AqdqNadh-Ubiquinone Oxidoreductase 18 Kda Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix SideThe Interaction With Bcap31 Mediates Mitochondria Localization |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial antibodyAnti-Complex I-18 Kda antibodyAnti-Ci-18 Kda antibodyAnti-Complex I-Aqdq antibodyAnti-Ci-Aqdq antibodyAnti-Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit antibodyAnti-NDUFS4 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cell pools, using [KD Validated] GPX4 Rabbit monoclonal antibody (STJ11101303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90216/359234/STJ11101303_1__60787.1713123659.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cell pools, using [KD Validated] GPX4 Rabbit monoclonal antibody (STJ11101303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
