• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from COLO, Jurkat, HeLa, HepG2, and HUVEC cells, using NDUFB10 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using NDUFB10 Polyclonal Antibody

Anti-NDUFB10 antibody (63-112) (STJ94378)

SKU:
STJ94378

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NADH dehydrogenase 1 beta subcomplex subunit 10 (63-112) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB10
Gene ID: 4716
Uniprot ID: NDUBA_HUMAN
Immunogen Region: 63-112
Specificity: NDUFB10 Polyclonal Antibody detects endogenous levels of NDUFB10 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NDUFB10. AA range:63-112
Post Translational Modifications The formation of intramolecular disulfide bonds is assisted by CHCHD4 and ensures folding, import into the mitochondrion and is required for the function in mitochondrial respiratory chain complex I assembly.
Function Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 10
Complex I-Pdsw
Ci-Pdsw
Nadh-Ubiquinone Oxidoreductase Pdsw Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 10 antibody
Anti-Complex I-Pdsw antibody
Anti-Ci-Pdsw antibody
Anti-Nadh-Ubiquinone Oxidoreductase Pdsw Subunit antibody
Anti-NDUFB10 antibody

Information sourced from Uniprot.org

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