• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g NDUFB10 antibody (STJ26523). Western blot was performed from the immunoprecipitate using NDUFB10 antibody (STJ26523) at a dilution of 1:1000.
  • Western blot analysis of various lysates using NDUFB10 Rabbit polyclonal antibody (STJ26523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NDUFB10 antibody (1-172) (STJ26523)

SKU:
STJ26523

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFB10 (1-172) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB10
Gene ID: 4716
Uniprot ID: NDUBA_HUMAN
Immunogen Region: 1-172
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1).
Immunogen Sequence: MPDSWDKDVYPEPPRRTPVQ PNPIVYMMKAFDLIVDRPVT LVREFIERQHAKNRYYYYHR QYRRVPDITECKEEDIMCMY EAEMQWKRDYKVDQEIINIM QDRLKACQQREGQNYQQNCI KEVEQFTQVAKAYQDRYQDL GAYSSARKCLAKQRQRMLQE RKAAKEAAAATS
Post Translational Modifications The formation of intramolecular disulfide bonds is assisted by CHCHD4 and ensures folding, import into the mitochondrion and is required for the function in mitochondrial respiratory chain complex I assembly.
Function Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 10
Complex I-Pdsw
Ci-Pdsw
Nadh-Ubiquinone Oxidoreductase Pdsw Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 10 antibody
Anti-Complex I-Pdsw antibody
Anti-Ci-Pdsw antibody
Anti-Nadh-Ubiquinone Oxidoreductase Pdsw Subunit antibody
Anti-NDUFB10 antibody

Information sourced from Uniprot.org

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