Anti-NDUFAB1 antibody (50-156) (STJ116863)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NDUFAB1 (50-156) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDUFAB1 |
| Gene ID | 4706 |
| Uniprot ID | ACPM_HUMAN |
| Immunogen Region | 50-156 |
| Immunogen Sequence | PALVLAQVPGRVTQLCRQYS DMPPLTLEGIQDRVLYVLKL YDKIDPEKLSVNSHFMKDLG LDSLDQVEIIMAMEDEFGFE IPDIDAEKLMCPQEIVDYIA DKKDVYE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-156 of human NDUFAB1 (NP_004994.1). |
Additional Info
| Post Translational Modifications | Phosphopantetheinylation at Ser-112 is essential for interactions with LYR motif-containing proteins. |
| Function | Carrier of the growing fatty acid chain in fatty acid biosynthesis. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. Accessory protein, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with LYRM4, the stability and the cysteine desulfurase activity of NFS1 and participates in the 2Fe-2S clusters assembly on the scaffolding protein ISCU. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a 2Fe-2S cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the 2Fe-2S cluster assembly. Finally, the 2Fe-2S cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5. |
| Protein Name | Acyl Carrier Protein - MitochondrialAcpCi-SdapNadh-Ubiquinone Oxidoreductase 9.6 Kda Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198Reactome: R-HSA-77289Reactome: R-HSA-9857492 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Acyl Carrier Protein - Mitochondrial antibodyAnti-Acp antibodyAnti-Ci-Sdap antibodyAnti-Nadh-Ubiquinone Oxidoreductase 9.6 Kda Subunit antibodyAnti-NDUFAB1 antibody |
Information sourced from Uniprot.org