• Western blot analysis of extracts of various cell lines, using NCOA3 antibody (STJ119458) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.

Anti-NCOA3 antibody (STJ119458)


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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-NCOA3 antibody is suitable for use in Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Gene ID: 8202
Gene Symbol: NCOA3
Immunogen: A synthetic Peptide of human NCOA3
Tissue Specificity Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Post Translational Modifications Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function. Methylated by CARM1. Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.
Function Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Protein Name Nuclear Receptor Coactivator 3
Amplified In Breast Cancer 1 Protein
Cbp-Interacting Protein
Class E Basic Helix-Loop-Helix Protein 42
Receptor-Associated Coactivator 3
Steroid Receptor Coactivator Protein 3
Thyroid Hormone Receptor Activator Molecule 1
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381340
Reactome: R-HSA-5617472
Reactome: R-HSA-5687128
Reactome: R-HSA-9018519
Cellular Localisation Cytoplasm
Mainly Cytoplasmic And Weakly Nuclear
Upon Tnf Activation And Subsequent Phosphorylation
It Translocates From The Cytoplasm To The Nucleus
Alternative Antibody Names Anti-Nuclear Receptor Coactivator 3 antibody
Anti-Ncoa-3 antibody
Anti-Actr antibody
Anti-Amplified In Breast Cancer 1 Protein antibody
Anti-Aib-1 antibody
Anti-Cbp-Interacting Protein antibody
Anti-Pcip antibody
Anti-Class E Basic Helix-Loop-Helix Protein 42 antibody
Anti-Bhlhe42 antibody
Anti-Receptor-Associated Coactivator 3 antibody
Anti-Rac-3 antibody
Anti-Steroid Receptor Coactivator Protein 3 antibody
Anti-Src-3 antibody
Anti-Thyroid Hormone Receptor Activator Molecule 1 antibody
Anti-Tram-1 antibody
Anti-NCOA3 antibody
Anti-AIB1 antibody
Anti-BHLHE42 antibody
Anti-RAC3 antibody
Anti-TRAM1 antibody

Information sourced from Uniprot.org

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