| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S0MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NCK1 |
| Gene ID: | 4690 |
| Uniprot ID: | NCK1_HUMAN |
| Immunogen Region: | 200-300 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NCK11 (P16333). |
| Immunogen Sequence: | PFSSSNDEELNFEKGDVMDV IEKPENDPEWWKCRKINGMV GLVPKNYVTVMQNNPLTSGL EPSPPQCDYIRPSLTGKFAG NPWYYGKVTRHQAEMALNER G |
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB. |
| Function | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. |
| Protein Name | Sh2/Sh3 Adapter Protein Nck1 Cytoplasmic Protein Nck1 Nck Adapter Protein 1 Nck-1 Sh2/Sh3 Adapter Protein Nck-Alpha |
| Database Links | Reactome: R-HSA-186763 Reactome: R-HSA-202433 Reactome: R-HSA-2029482 Reactome: R-HSA-373753 Reactome: R-HSA-418885 Reactome: R-HSA-428540 Reactome: R-HSA-4420097 Reactome: R-HSA-5663213 Reactome: R-HSA-9013420 Reactome: R-HSA-9013424 Reactome: R-HSA-9664422 Reactome: R-HSA-9679191 Reactome: R-HSA-9833482 Reactome: R-HSA-983695 |
| Cellular Localisation | Cytoplasm Endoplasmic Reticulum Nucleus Mostly Cytoplasmic But Shuttles Between The Cytoplasm And The Nucleus Import Into The Nucleus Requires The Interaction With Socs7 Predominantly Nuclear Following Genotoxic Stresses Such As Uv Irradiation Hydroxyurea Or Mitomycin C Treatments |
| Alternative Antibody Names | Anti-Sh2/Sh3 Adapter Protein Nck1 antibody Anti-Cytoplasmic Protein Nck1 antibody Anti-Nck Adapter Protein 1 antibody Anti-Nck-1 antibody Anti-Sh2/Sh3 Adapter Protein Nck-Alpha antibody Anti-NCK1 antibody Anti-NCK antibody |
Information sourced from Uniprot.org