• Immunofluorescence analysis of U2OS cells using NCAPD2 Rabbit polyclonal antibody (STJ11100366) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using NCAPD2 Rabbit polyclonal antibody (STJ11100366) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using NCAPD2 Rabbit polyclonal antibody (STJ11100366) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NCAPD2 Rabbit polyclonal antibody (STJ11100366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NCAPD2 antibody (1250-1350) (STJ11100366)

SKU:
STJ11100366

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCAPD2 (1250-1350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCAPD2
Gene ID: 9918
Uniprot ID: CND1_HUMAN
Immunogen Region: 1250-1350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1250-1350 of human NCAPD2 (NP_055680.3).
Immunogen Sequence: FGDKLSDESIFSAFLSVVGK LRRGAKPEGKAIIDEFEQKL RACHTRGLDGIKELEIGQAG SQRAPSAKKPSTGSRYQPLA STASDNDFVTPEPRRTTRRH P
Post Translational Modifications Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPH and NCAPG subunits, activates the condensin complex and is required for chromosome condensation.
Function Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.
Protein Name Condensin Complex Subunit 1
Chromosome Condensation-Related Smc-Associated Protein 1
Chromosome-Associated Protein D2
Hcap-D2
Non-Smc Condensin I Complex Subunit D2
Xcap-D2 Homolog
Database Links Reactome: R-HSA-2514853
Cellular Localisation Nucleus
Cytoplasm
Chromosome
In Interphase Cells
The Majority Of The Condensin Complex Is Found In The Cytoplasm
While A Minority Of The Complex Is Associated With Chromatin
A Subpopulation Of The Complex However Remains Associated With Chromosome Foci In Interphase Cells
During Mitosis
Most Of The Condensin Complex Is Associated With The Chromatin
At The Onset Of Prophase
The Regulatory Subunits Of The Complex Are Phosphorylated By Cdk1
Leading To Condensin's Association With Chromosome Arms And To Chromosome Condensation
Dissociation From Chromosomes Is Observed In Late Telophase
Alternative Antibody Names Anti-Condensin Complex Subunit 1 antibody
Anti-Chromosome Condensation-Related Smc-Associated Protein 1 antibody
Anti-Chromosome-Associated Protein D2 antibody
Anti-Hcap-D2 antibody
Anti-Non-Smc Condensin I Complex Subunit D2 antibody
Anti-Xcap-D2 Homolog antibody
Anti-NCAPD2 antibody
Anti-CAPD2 antibody
Anti-CNAP1 antibody
Anti-KIAA0159 antibody

Information sourced from Uniprot.org

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