Western blot analysis of Rat lung, using NAPSA Rabbit polyclonal antibody (STJ11105353) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NAPSA antibody (64-190) (STJ11105353)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105353
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General Information

Short DescriptionRabbit polyclonal Napsin-A (64-190) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityRat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNAPSA
Gene ID9476
Uniprot IDNAPSA_HUMAN
Immunogen Region64-190
Immunogen SequenceKPIFVPLSNYRDVQYFGEIG LGTPPQNFTVAFDTGSSNLW VPSRRCHFFSVPCWLHHRFD PKASSSFQANGTKFAIQYGT GRVDGILSEDKLTIGGIKGA SVIFGEALWEPSLVFAFAHF DGILGLG
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 64-190 of human NAPSA (NP_004842.1).

Additional Info

Tissue Specificity Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Function May be involved in processing of pneumocyte surfactant precursors.
Protein Name Napsin-A
Aspartyl Protease 4
Asp4
Asp 4
Napsin-1
Ta01/Ta02
Database Links Reactome: R-HSA-5683826
Cellular Localisation Secreted
Alternative Antibody Names Anti-Napsin-A antibody
Anti-Aspartyl Protease 4 antibody
Anti-Asp4 antibody
Anti-Asp 4 antibody
Anti-Napsin-1 antibody
Anti-Ta01/Ta02 antibody
Anti-NAPSA antibody
Anti-NAP1 antibody
Anti-NAPA antibody

Information sourced from Uniprot.org

Citations

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