| Short Description : | Rabbit polyclonal NAPRT (309-538) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NAPRT |
| Gene ID: | 93100 |
| Uniprot ID: | PNCB_HUMAN |
| Immunogen Region: | 309-538 |
| Immunogen Sequence: | ELGYRAVGVRLDSGDLLQQA QEIRKVFRAAAAQFQVPWLE SVLIVVSNNIDEEALARLAQ EGSEVNVIGIGTSVVTCPQQ PSLGGVYKLVAVGGQPRMKL TEDPEKQTLPGSKAAFRLLG SDGSPLMDMLQLAEEPVPQA GQELRVWPPGAQEPCTVRPA QVEPLLRLCLQQGQLCEPLP SLAESRALAQLSLSRLSPEH RRLRSPAQYQVVLSERLQAL VNSLCAGQSP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 309-538 of human NAPRT1 (NP_660202.3). |
| Post Translational Modifications | Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release. |
| Function | Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis. |
| Protein Name | Nicotinate PhosphoribosyltransferaseNaprtaseFha-Hit-Interacting ProteinNicotinate Phosphoribosyltransferase Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-197264Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Nicotinate Phosphoribosyltransferase antibodyAnti-Naprtase antibodyAnti-Fha-Hit-Interacting Protein antibodyAnti-Nicotinate Phosphoribosyltransferase Domain-Containing Protein 1 antibodyAnti-NAPRT antibodyAnti-FHIP antibodyAnti-NAPRT1 antibody |
Information sourced from Uniprot.org
