• Immunohistochemistry analysis of paraffin-embedded human gastric using NAA60 antibody (STJ29523) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using NAA60 antibody (STJ29523) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using NAA60 antibody (STJ29523) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of 293F, using NAA60 Rabbit polyclonal antibody (STJ29523) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NAA60 antibody (1-184) (STJ29523)

SKU:
STJ29523

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAA60 (1-184) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAA60
Gene ID: 79903
Uniprot ID: NAA60_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human NAA60 (NP_001077070.1).
Immunogen Sequence: MTEVVPSSALSEVSLRLLCH DDIDTVKHLCGDWFPIEYPD SWYRDITSNKKFFSLAATYR GAIVGMIVAEIKNRTKIHKE DGDILASNFSVDTQVAYILS LGVVKEFRKHGIGSLLLESL KDHISTTAQDHCKAIYLHVL TTNNTAINFYENRDFKQHHY LPYYYSIRGVLKDGFTYVLY INGG
Post Translational Modifications Acetylated: autoacetylation is required for optimal acetyltransferase activity.
Function N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity.such results are however unclear in vivo and would require additional experimental evidences.
Protein Name N-Alpha-Acetyltransferase 60
Hnaa60
Histone Acetyltransferase Type B Protein 4
Hat4
N-Acetyltransferase 15
N-Alpha-Acetyltransferase F
Natf
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Probably Forms An Intramembrane Hairpin-Like Structure In The Membrane
Alternative Antibody Names Anti-N-Alpha-Acetyltransferase 60 antibody
Anti-Hnaa60 antibody
Anti-Histone Acetyltransferase Type B Protein 4 antibody
Anti-Hat4 antibody
Anti-N-Acetyltransferase 15 antibody
Anti-N-Alpha-Acetyltransferase F antibody
Anti-Natf antibody
Anti-NAA60 antibody
Anti-HAT4 antibody
Anti-NAT15 antibody
Anti-UNQ2771 antibody
Anti-PRO7155 antibody

Information sourced from Uniprot.org

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