Anti-N4BP1 antibody (383-432) (STJ94327)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432
General Information
| Short Description | Rabbit polyclonal anti-NEDD4-binding protein 1 (383-432) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | N4BP1 |
| Gene ID | 9683 |
| Uniprot ID | N4BP1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432 |
| Immunogen Region | 383-432 |
| Specificity | N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein. |
Additional Info
| Tissue Specificity | Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. |
| Post Translational Modifications | Proteolytically cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation. Mainly cleaved at Asp-490 by CASP8. Cleaved by caspase-like protein MALT1 in T-cells following TCR-mediated activation, leading to its inactivation and subsequent viral reactivation during HIV-1 infection. Mono- and polyubiquitinated on the CoCUN region. Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies. |
| Function | Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation. Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults. In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines. Acts as a restriction factor against some viruses, such as HIV-1: restricts HIV-1 replication by binding to HIV-1 mRNAs and mediating their degradation via its ribonuclease activity. Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH: acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. |
| Protein Name | Nedd4-Binding Protein 1N4bp1 |
| Database Links | Reactome: R-HSA-9758274 |
| Cellular Localisation | CytoplasmCytosolNucleusNucleolusPml BodyPrimarily Localizes To The NucleolusAlso Localizes To The Pml Nuclear BodiesWhen Desumoylated |
| Alternative Antibody Names | Anti-Nedd4-Binding Protein 1 antibodyAnti-N4bp1 antibodyAnti-N4BP1 antibodyAnti-KIAA0615 antibody |
Information sourced from Uniprot.org