• Western blot analysis of extracts of RD cels, using MyoD1 antibody (STJ11104689) at1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-MYOD1 antibody (100-200) [S4689RM] (STJ11104689)

SKU:
STJ11104689

€183.13 - €799.38
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Myoblast Determination Protein 1 (100-200) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4689RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYOD1
Gene ID: 4654
Uniprot ID: MYOD1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MyoD1 (NP_002469.2).
Immunogen Sequence: ACKRKTTNADRRKAATMRER RRLSKVNEAFETLKRCTSSN PNQRLPKVEILRNAIRYIEG LQALLRDQDAAPPGAAAAFY APGPLPPGRGGEHYSGDSDA S
Post Translational Modifications Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function. Ubiquitinated on the N-terminus.which is required for proteasomal degradation. Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Protein Name Myoblast Determination Protein 1
Class C Basic Helix-Loop-Helix Protein 1
Bhlhc1
Myogenic Factor 3
Myf-3
Database Links Reactome: R-HSA-525793
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Myoblast Determination Protein 1 antibody
Anti-Class C Basic Helix-Loop-Helix Protein 1 antibody
Anti-Bhlhc1 antibody
Anti-Myogenic Factor 3 antibody
Anti-Myf-3 antibody
Anti-MYOD1 antibody
Anti-BHLHC1 antibody
Anti-MYF3 antibody
Anti-MYOD antibody

Information sourced from Uniprot.org

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