• Immunofluorescence analysis of U-2 OS cells using MTHFD2 antibody (STJ112422) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using MTHFD2 antibody (STJ112422) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MTHFD2 antibody (STJ112422) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using MTHFD2 antibody (STJ112422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using MTHFD2 antibody (STJ112422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using MTHFD2 antibody (STJ112422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using MTHFD2 antibody (STJ112422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (STJ112422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MTHFD2 antibody (40-250) (STJ112422)

SKU:
STJ112422

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTHFD2 (40-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTHFD2
Gene ID: 10797
Uniprot ID: MTDC_HUMAN
Immunogen Region: 40-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
Immunogen Sequence: ISGRKLAQQIKQEVRQEVEE WVASGNKRPHLSVILVGENP ASHSYVLNKTRAAAVVGINS ETIMKPASISEEELLNLINK LNNDDNVDGLLVQLPLPEHI DERRICNAVSPDKDVDGFHV INVGRMCLDQYSMLPATPWG VWEIIKRTGIPTLGKNVVVA GRSKNVGMPIAMLLHTDGAH ERPGGDATVTISHRYTPKEQ LKKHTILADIV
Function Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Protein Name Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase - Mitochondrial Includes - Nad-Dependent Methylenetetrahydrofolate Dehydrogenase - Methenyltetrahydrofolate Cyclohydrolase
Database Links Reactome: R-HSA-196757
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase - Mitochondrial Includes - Nad-Dependent Methylenetetrahydrofolate Dehydrogenase - Methenyltetrahydrofolate Cyclohydrolase antibody
Anti-MTHFD2 antibody
Anti-NMDMC antibody

Information sourced from Uniprot.org

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