| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WAS (60-250) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WAS |
| Gene ID: |
7454 |
| Uniprot ID: |
WASP_HUMAN |
| Immunogen Region: |
60-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WASPP (NP_000368.1). |
| Immunogen Sequence: |
GAEHWTKEHCGAVCFVKDNP QKSYFIRLYGLQAGRLLWEQ ELYSQLVYSTPTPFFHTFAG DDCQAGLNFADEDEAQAFRA LVQEKIQKRNQRQSGDRRQL PPPPTPANEERRGGLPPLPL HPGGDQGGPPVGPLSLGLAT VDIQNPDITSSRYRGLPAPG PSPADKKRSGKKKISKADIG APSGFKHVSHV |
| Tissue Specificity | Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. |
| Post Translational Modifications | Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation. |
| Function | Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). |
| Protein Name | Actin Nucleation-Promoting Factor WasWiskott-Aldrich Syndrome ProteinWasp |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-2029482Reactome: R-HSA-5663213Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus |
| Alternative Antibody Names | Anti-Actin Nucleation-Promoting Factor Was antibodyAnti-Wiskott-Aldrich Syndrome Protein antibodyAnti-Wasp antibodyAnti-WAS antibodyAnti-IMD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
