• Immunofluorescence analysis of U2OS cells using MTDH antibody (STJ29926) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MTDH Rabbit polyclonal antibody (STJ29926) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using MTDH Rabbit polyclonal antibody (STJ29926) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using MTDH Rabbit polyclonal antibody (STJ29926) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MTDH Rabbit polyclonal antibody (STJ29926) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of PC-12, using MTDH antibody (STJ29926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 7s.

Anti-MTDH antibody (1-260) (STJ29926)

SKU:
STJ29926

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTDH (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTDH
Gene ID: 92140
Uniprot ID: LYRIC_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MTDH (NP_848927.2).
Immunogen Sequence: MAARSWQDELAQQAEEGSAR LREMLSVGLGFLRTELGLDL GLEPKRYPGWVILVGTGALG LLLLFLLGYGWAAACAGARK KRRSPPRKREEAAAVPAAAP DDLALLKNLRSEEQKKKNRK KLSEKPKPNGRTVEVAEGEA VRTPQSVTAKQPPEIDKKNE KSKKNKKKSKSDAKAVQNSS RHDGKEVDEGAWETKISHRE KRQQRKRDKVLTDSGSLDST IPGIENTITVTTEQLTTAS
Tissue Specificity Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
Function Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
Protein Name Protein Lyric
3d3/Lyric
Astrocyte Elevated Gene-1 Protein
Aeg-1
Lysine-Rich Ceacam1 Co-Isolated Protein
Metadherin
Metastasis Adhesion Protein
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Nucleus Membrane
Cell Junction
Tight Junction
Nucleus
Nucleolus
Cytoplasm
Perinuclear Region
In Epithelial Cells
Recruited To Tight Junctions (Tj) During The Maturation Of The Tj Complexes
A Nucleolar Staining May Be Due To Nuclear Targeting Of An Isoform Lacking The Transmembrane Domain
Tnf-Alpha Causes Translocation From The Cytoplasm To The Nucleus
Alternative Antibody Names Anti-Protein Lyric antibody
Anti-3d3/Lyric antibody
Anti-Astrocyte Elevated Gene-1 Protein antibody
Anti-Aeg-1 antibody
Anti-Lysine-Rich Ceacam1 Co-Isolated Protein antibody
Anti-Metadherin antibody
Anti-Metastasis Adhesion Protein antibody
Anti-MTDH antibody
Anti-AEG1 antibody
Anti-LYRIC antibody

Information sourced from Uniprot.org

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