Western blot analysis of various lysates using MT-ND4 Rabbit pAb (STJ111982) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-MT-ND4 antibody (413-429) (STJ111982)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111982
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General Information

Short DescriptionRabbit polyclonal MT-ND4 (413-429) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMtnd4
Gene ID17719
Uniprot IDNU4M_MOUSE
Immunogen Region413-429
Immunogen SequenceLMASLANLALPPSINLMGEL FITMSLFSWSNFTIILMGIN IIITGMYSMYMIITTQRGKL TNHMINLQPSHTRELTLMAL HMIPLILLTTSPKLITGLTM
SpecificityA synthetic peptide corresponding to a sequence within amino acids 360-459 of mouse MT-ND4 (NP_904337.1).

Additional Info

Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 4
Nadh Dehydrogenase Subunit 4
Database Links Reactome: R-MMU-611105
Reactome: -MMU-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 4 antibody
Anti-Nadh Dehydrogenase Subunit 4 antibody
Anti-Mtnd4 antibody
Anti-mt-Nd4 antibody
Anti-Nd4 antibody

Information sourced from Uniprot.org

Citations

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