• Western blot analysis of extracts of various cell lines, using MT-ND4 antibody (STJ111982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using MT-ND4 antibody (STJ111982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MT-ND4 antibody (413-429) (STJ111982)

SKU:
STJ111982

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ND4 (413-429) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mtnd4
Gene ID: 17719
Uniprot ID: NU4M_MOUSE
Immunogen Region: 413-429
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 413-429 of mouse MT-ND4 (NP_904337.1).
Immunogen Sequence: TTQRGKLTNHMINLQPS
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 4
Nadh Dehydrogenase Subunit 4
Database Links Reactome: R-MMU-611105
Reactome: -MMU-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 4 antibody
Anti-Nadh Dehydrogenase Subunit 4 antibody
Anti-Mtnd4 antibody
Anti-mt-Nd4 antibody
Anti-Nd4 antibody

Information sourced from Uniprot.org

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