• Western blot analysis of extracts of various cell lines, using ATP6 antibody (STJ110492) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using ATP6 Rabbit polyclonal antibody (STJ110492) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using ATP6 antibody (STJ110492) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit polyclonal antibody (STJ110492) at 1:2000；1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-MT-ATP6 antibody (209-226) (STJ110492)

SKU:
STJ110492

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6 (209-226) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mtatp6
Gene ID: 17705
Uniprot ID: ATP6_MOUSE
Immunogen Region: 209-226
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 209-226 of mouse ATP6 (NP_904333.1).
Immunogen Sequence: IQAYVFTLLVSLYLHDNT
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel.it may play a direct role in the translocation of protons across the membrane.
Protein Name Atp Synthase Subunit A
F-Atpase Protein 6
Database Links Reactome: R-MMU-163210
Reactome: -MMU-8949613
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp Synthase Subunit A antibody
Anti-F-Atpase Protein 6 antibody
Anti-Mtatp6 antibody
Anti-Atp6 antibody
Anti-Atpase6 antibody
Anti-mt-Atp6 antibody

Information sourced from Uniprot.org

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