• Immunofluorescence analysis of HeLa cells using MSRB1 Rabbit polyclonal antibody (STJ28820). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MSRB1 in paraffin-embedded Mouse testis tissue using MSRB1 Rabbit polyclonal antibody (STJ28820) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MSRB1 in paraffin-embedded Rat testis tissue using MSRB1 Rabbit polyclonal antibody (STJ28820) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MSRB1 in paraffin-embedded Human colon tissue using MSRB1 Rabbit polyclonal antibody (STJ28820) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MSRB1 Rabbit polyclonal antibody (STJ28820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MSRB1 antibody (1-94) (STJ28820)

SKU:
STJ28820

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSRB1 (1-94) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSRB1
Gene ID: 51734
Uniprot ID: MSRB1_HUMAN
Immunogen Region: 1-94
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human MSRB1 (NP_057416.1).
Immunogen Sequence: MSFCSFFGGEVFQNHFEPGV YVCAKCGYELFSSRSKYAHS SPWPAFTETIHADSVAKRPE HNRSEALKVSCGKCGNGLGH EFLNDGPKPGQSRF
Post Translational Modifications Truncated MSRB1/SEPX1 proteins produced by failed UGA/Sec decoding are ubiquitinated by some Cul2-RING E3 ubiquitin-protein ligase complexes (containing either PRAME, PRAMF6, PRAMF9 or FEM1C as substrate-recognition component).
Function Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by MICALs (MICAL1, MICAL2 or MICAL3) on actin, thereby promoting filament repolymerization. Plays a role in innate immunity by reducing oxidized actin, leading to actin repolymerization in macrophages.
Protein Name Methionine-R-Sulfoxide Reductase B1
Msrb1
Selenoprotein X
Selx
Database Links Reactome: R-HSA-5676934
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Alternative Antibody Names Anti-Methionine-R-Sulfoxide Reductase B1 antibody
Anti-Msrb1 antibody
Anti-Selenoprotein X antibody
Anti-Selx antibody
Anti-MSRB1 antibody
Anti-SEPX1 antibody
Anti-HSPC270 antibody

Information sourced from Uniprot.org

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