• Western blot analysis of extracts of OVCAR3 cells, using Mesothelin antibody (STJ116034) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer (200X) using Mesothelin rabbit polyclonal antibody (STJ116034) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer (400X) using Mesothelin rabbit polyclonal antibody (STJ116034) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil (200X) using Mesothelin rabbit polyclonal antibody (STJ116034) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil (400X) using Mesothelin rabbit polyclonal antibody (STJ116034) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MSLN antibody (296-606) (STJ116034)

SKU:
STJ116034

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSLN (296-606) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSLN
Gene ID: 10232
Uniprot ID: MSLN_HUMAN
Immunogen Region: 296-606
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2).
Immunogen Sequence: EVEKTACPSGKKAREIDESL IFYKKWELEACVDAALLATQ MDRVNAIPFTYEQLDVLKHK LDELYPQGYPESVIQHLGYL FLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQAPRRP LPQVATLIDRFVKGRGQLDK DTLDTLTAFYPGYLCSLSPE ELSSVPPSSIWAVRPQDLDT CDPRQLDVLYPKARLAFQNM NGSEYFVKIQSFLGGAPTED LKALSQQNVSMDLATFMKL
Tissue Specificity Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Post Translational Modifications Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Function Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Protein Name Mesothelin
Cak1 Antigen
Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor
Mpf - Mesothelin - Cleaved Form
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Megakaryocyte-Potentiating Factor: Secreted
Isoform 3: Secreted
Alternative Antibody Names Anti-Mesothelin antibody
Anti-Cak1 Antigen antibody
Anti-Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor antibody
Anti-Mpf - Mesothelin - Cleaved Form antibody
Anti-MSLN antibody
Anti-MPF antibody

Information sourced from Uniprot.org

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