| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Myocardin-related transcription factor A (10-59) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRTFA |
| Gene ID: | 57591 |
| Uniprot ID: | MRTFA_HUMAN |
| Immunogen Region: | 10-59 |
| Specificity: | MRTF-A Polyclonal Antibody detects endogenous levels of MRTF-A protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MKL1. AA range:10-59 |
| Tissue Specificity | Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain. Expressed in peripheral blood mononuclear cells (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-6 by Erk inhibits binding of globular actin (G-actin), unmasking the nuclear localization signal (NLS) and promoting nuclear import. |
| Function | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus. |
| Protein Name | Myocardin-Related Transcription Factor A Mrtf-A Mkl/Myocardin-Like Protein 1 Megakaryoblastic Leukemia 1 Protein Megakaryocytic Acute Leukemia Protein |
| Database Links | Reactome: R-HSA-3899300 Reactome: R-HSA-5663220 |
| Cellular Localisation | Cytoplasm Nucleus Subcellular Location Is Tightly Regulated By Actin Both In Cytoplasm And Nucleus: High Levels Of G-Actin In The Nucleus Observed During Serum Deprivation Lead To Low Levels Of Nuclear Mrtfa While Reduced Levels Of Nuclear G-Actin Result In Accumulation Of Mrtfa In The Nucleus G-Actin-Binding In The Cytoplasm Inhibits Nuclear Import By Masking The Nuclear Localization Signal (Nls) In Contrast Binding To Nuclear Globular Actin (G-Actin) Promotes Nuclear Export To The Cytoplasm Nuclear Localization Is Regulated By Mical2 Which Mediates Depolymerization Of Nuclear Actin Which Decreases Nuclear G-Actin Pool Thereby Promoting Retention Of Mrtfa In The Nucleus And Subsequent Formation Of An Active Complex With Srf Nuclear Import Is Mediated By Importins Kpna4 And Kpnb1 |
| Alternative Antibody Names | Anti-Myocardin-Related Transcription Factor A antibody Anti-Mrtf-A antibody Anti-Mkl/Myocardin-Like Protein 1 antibody Anti-Megakaryoblastic Leukemia 1 Protein antibody Anti-Megakaryocytic Acute Leukemia Protein antibody Anti-MRTFA antibody Anti-KIAA1438 antibody Anti-MAL antibody Anti-MKL1 antibody |
Information sourced from Uniprot.org