• Immunofluorescence analysis of MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from wild type (WT) and MRPL9 knockdown (KD) HeLa cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MRPL9 antibody (53-267) (STJ11105615)

SKU:
STJ11105615

£129.50 - £642.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL9
Gene ID: 65005
Uniprot ID: RM09_HUMAN
Immunogen Region: 53-267
Specificity: Recombinant Protein corresponding to a sequence within amino acids 53-267 of human MRPL9 (NP_113608.1).
Immunogen Sequence: TVIVERWWKVPLAGEGRKPR LHRRHRVYKLVEDTKHRPKE NLELILTQSVENVGVRGDLV SVKKSLGRNRLLPQGLAVYA SPENKKLFEEEKLLRQEGKL EKIQTKAGEATVKFLKSCRL EVGMKNNVKWELNPEIVARH FFKNLGVVVAPHTLKLPEEP ITRWGEYWCEVTVNGLDTVR VPMSVVNFEKPKTKRYKYWL AQQAAKAMAPTSPQI
Protein Name Large Ribosomal Subunit Protein Bl9m
39s Ribosomal Protein L9 - Mitochondrial
L9mt
Mrp-L9
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Bl9m antibody
Anti-39s Ribosomal Protein L9 - Mitochondrial antibody
Anti-L9mt antibody
Anti-Mrp-L9 antibody
Anti-MRPL9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance