• Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from A549 cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MRPL53 antibody (1-112) (STJ11105622)

SKU:
STJ11105622

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL53
Gene ID: 116540
Uniprot ID: RM53_HUMAN
Immunogen Region: 1-112
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of human MRPL53 ( NP_444278.1 ).
Immunogen Sequence: MAAALARLGLRPVKQVRVQF CPFEKNVESTRTFLQTVSSE KVRSTNLNCSVIADVRHDGS EPCVDVLFGDGHRLIMRGAH LTALEMLTAFASHIRARDAA GSGDKPGADTGR
Protein Name Large Ribosomal Subunit Protein Ml53
39s Ribosomal Protein L53 - Mitochondrial
L53mt
Mrp-L53
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ml53 antibody
Anti-39s Ribosomal Protein L53 - Mitochondrial antibody
Anti-L53mt antibody
Anti-Mrp-L53 antibody
Anti-MRPL53 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance