Anti-MRPL32 antibody (101-150) (STJ94222)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human MRPL32. AA range:101-150
General Information
| Short Description | Rabbit polyclonal anti-Large ribosomal subunit protein bL32m (101-150) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MRPL32 |
| Gene ID | 64983 |
| Uniprot ID | RM32_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL32. AA range:101-150 |
| Immunogen Region | 101-150 |
| Specificity | MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein. |
Additional Info
| Post Translational Modifications | MRPL32 precursor is processed by the m-AAA protease (composed of AFG3L2 and SPG7), which cleaves the N-terminal transit peptide. Cleavage by the m-AAA protease takes place prior to assembly into the large subunit, an essential step for mitochondrial ribosome (mitoribosome) assembly. Proper processing by the m-AAA protease is dependent on the zinc-binding region within the tightly folded C-terminal domain of MRPL32: zinc-dependent folding halts degradation initiated from the N-terminus and triggers the release of mature MRPL32. |
| Function | Component of the mitochondrial large ribosomal subunit (mt-LSU). The mitochondrial ribosome (mitoribosome) is a large ribonucleoprotein complex responsible for the synthesis of proteins inside mitochondria. |
| Protein Name | Large Ribosomal Subunit Protein Bl32m39s Ribosomal Protein L32 - MitochondrialL32mtMrp-L32 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl32m antibodyAnti-39s Ribosomal Protein L32 - Mitochondrial antibodyAnti-L32mt antibodyAnti-Mrp-L32 antibodyAnti-MRPL32 antibodyAnti-HSPC283 antibody |
Information sourced from Uniprot.org