• Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K9 antibody (STJ23984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (STJ23984).
  • Immunohistochemistry analysis of paraffin-embedded human colon using MonoMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23984) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23984) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 antibody (STJ23984). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-K9 antibody (STJ23984) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 antibody (STJ23984) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 antibody (STJ23984) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K9 antibody (STJ23984) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Anti-Mono-Methyl-Histone H3-K9 antibody (STJ23984)

Anti-Mono-Methyl-Histone H3-K9 antibody (STJ23984)

SKU:
STJ23984

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mono-Methyl-Histone H3-K9 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic monomethylated peptide around K9 of human histone H3 (NP_003520.1).
Immunogen Sequence: ARKST
Background This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Information sourced from Uniprot.org

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