• Immunohistochemistry analysis of MOB4 in paraffin-embedded human oophoroma using MOB4 Rabbit polyclonal antibody (STJ26745) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MOB4 in paraffin-embedded rat ovary using MOB4 Rabbit polyclonal antibody (STJ26745) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MOB4 Rabbit polyclonal antibody (STJ26745) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MOB4 antibody (1-225) (STJ26745)

SKU:
STJ26745

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MOB4
Gene ID: 25843
Uniprot ID: PHOCN_HUMAN
Immunogen Region: 1-225
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human MOB4 (NP_056202.2).
Immunogen Sequence: MVMAEGTAVLRRNRPGTKAQ DFYNWPDESFDEMDSTLAVQ QYIQQNIRADCSNIDKILEP PEGQDEGVWKYEHLRQFCLE LNGLAVKLQSECHPDTCTQM TATEQWIFLCAAHKTPKECP AIDYTRHTLDGAACLLNSNK YFPSRVSIKESSVAKLGSVC RRIYRIFSHAYFHHRQIFDE YENETFLCHRFTKFVMKYNL MSKDNLIVPILEEEVQNSVS GESEA
Post Translational Modifications Phosphorylated on serine residues.
Function Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.
Protein Name Mob-Like Protein Phocein
2c4d
Class Ii Mmob1
Mob1 Homolog 3
Mob3
Mps One Binder Kinase Activator-Like 3
Preimplantation Protein 3
Cellular Localisation Cytoplasm
Perinuclear Region
Membrane
Peripheral Membrane Protein
Golgi Apparatus
Golgi Stack Membrane
In A Perinuclear Punctate Pattern
Associated With Membranes And The Golgi Stacks
Alternative Antibody Names Anti-Mob-Like Protein Phocein antibody
Anti-2c4d antibody
Anti-Class Ii Mmob1 antibody
Anti-Mob1 Homolog 3 antibody
Anti-Mob3 antibody
Anti-Mps One Binder Kinase Activator-Like 3 antibody
Anti-Preimplantation Protein 3 antibody
Anti-MOB4 antibody
Anti-MOB3 antibody
Anti-MOBKL3 antibody
Anti-PHOCN antibody
Anti-PREI3 antibody
Anti-CGI-95 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance