• Western blot analysis of various lysates, using MOB1A/MOB1B rabbit polyclonal antibody (STJ11100203) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates, using MOB1A/MOB2B rabbit polyclonal antibody (STJ11100203) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using MOB1A/MOB1B antibody (STJ11100203) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MOB1A/MOB1B antibody (150-250) (STJ11100203)

SKU:
STJ11100203

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MOB1A/MOB1B (150-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human MOB1A/MOB1B (NP_060691.2/NP_775739.1).
Immunogen Sequence: TILKRLFRVYAHIYHQHFDS VMQLQEEAHLNTSFKHFIFF VQEFNLIDRRELAPLQELIE KLGSKDR/MSFLFGSRSSKT FKPKKNIPEGSHQYELLKHA E
Background This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Information sourced from Uniprot.org

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