• Immunohistochemistry analysis of paraffin-embedded human liver cancer using MMP8 Rabbit polyclonal antibody (STJ24616) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MMP8 antibody (STJ24616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MMP8 antibody (101-467) (STJ24616)

SKU:
STJ24616

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP8 (101-467) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP8
Gene ID: 4317
Uniprot ID: MMP8_HUMAN
Immunogen Region: 101-467
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 101-467 of human MMP8 (NP_002415.1).
Immunogen Sequence: LTPGNPKWERTNLTYRIRNY TPQLSEAEVERAIKDAFELW SVASPLIFTRISQGEADINI AFYQRDHGDNSPFDGPNGIL AHAFQPGQGIGGDAHFDAEE TWTNTSANYNLFLVAAHEFG HSLGLAHSSDPGALMYPNYA FRETSNYSLPQDDIDGIQAI YGLSSNPIQPTGPSTPKPCD PSLTFDAITTLRGEILFFKD RYFWRRHPQLQRVEMNFISL FWPSLPTGIQAAYEDFDRD
Tissue Specificity Neutrophils.
Function Can degrade fibrillar type I, II, and III collagens.
Protein Name Neutrophil Collagenase
Matrix Metalloproteinase-8
Mmp-8
Pmnl Collagenase
Pmnl-Cl
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-6798695
Cellular Localisation Cytoplasmic Granule
Secreted
Extracellular Space
Extracellular Matrix
Stored In Intracellular Granules
Alternative Antibody Names Anti-Neutrophil Collagenase antibody
Anti-Matrix Metalloproteinase-8 antibody
Anti-Mmp-8 antibody
Anti-Pmnl Collagenase antibody
Anti-Pmnl-Cl antibody
Anti-MMP8 antibody
Anti-CLG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance