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Anti-MMP12 antibody (200-300) (STJ111106)

Western blot analysis of extracts of A-549 cells, using MMP12 antibody (STJ111106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using MMP12 rabbit polyclonal antibody (STJ111106) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MMP12 rabbit polyclonal antibody (STJ111106) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MMP12 antibody (200-300) (STJ111106)

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STJ111106

$151.88 - $705.38

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-MMP12 (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MMP12
Gene ID:	4321
Uniprot ID:	MMP12_HUMAN
Immunogen Region:	200-300
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MMP12 (NP_002417.2).
Immunogen Sequence:	DEFWTTHSGGTNLFLTAVHE IGHSLGLGHSSDPKAVMFPT YKYVDINTFRLSADDIRGIQ SLYGDPKENQRLPNPDNSEP ALCDPNLSFDAVTTVGNKIF F

Tissue Specificity	Found in alveolar macrophages but not in peripheral blood monocytes.
Function	May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Protein Name	Macrophage Metalloelastase Mme Macrophage Elastase Me Hme Matrix Metalloproteinase-12 Mmp-12
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1474228
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Macrophage Metalloelastase antibody Anti-Mme antibody Anti-Macrophage Elastase antibody Anti-Me antibody Anti-Hme antibody Anti-Matrix Metalloproteinase-12 antibody Anti-Mmp-12 antibody Anti-MMP12 antibody Anti-HME antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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