• Western blot analysis of extracts of A-549 cells, using MMP12 antibody (STJ111106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A-549 cells using MMP12 rabbit polyclonal antibody (STJ111106) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using MMP12 rabbit polyclonal antibody (STJ111106) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MMP12 antibody (200-300) (STJ111106)

SKU:
STJ111106

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP12
Gene ID: 4321
Uniprot ID: MMP12_HUMAN
Immunogen Region: 200-300
Specificity: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MMP12 (NP_002417.2).
Immunogen Sequence: DEFWTTHSGGTNLFLTAVHE IGHSLGLGHSSDPKAVMFPT YKYVDINTFRLSADDIRGIQ SLYGDPKENQRLPNPDNSEP ALCDPNLSFDAVTTVGNKIF F
Tissue Specificity Found in alveolar macrophages but not in peripheral blood monocytes.
Function May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Protein Name Macrophage Metalloelastase
Mme
Macrophage Elastase
Me
Hme
Matrix Metalloproteinase-12
Mmp-12
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Macrophage Metalloelastase antibody
Anti-Mme antibody
Anti-Macrophage Elastase antibody
Anti-Me antibody
Anti-Hme antibody
Anti-Matrix Metalloproteinase-12 antibody
Anti-Mmp-12 antibody
Anti-MMP12 antibody
Anti-HME antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance