• Immunofluorescence analysis of NIH-3T3 cells using MMP10 Rabbit polyclonal antibody (STJ24574) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MMP10 in paraffin-embedded mouse brain using MMP10 Rabbit polyclonal antibody (STJ24574) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MMP10 in paraffin-embedded rat kidney using MMP10 Rabbit polyclonal antibody (STJ24574) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MMP10 Rabbit polyclonal antibody (STJ24574) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MMP10 antibody (237-476) (STJ24574)

SKU:
STJ24574

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP10 (237-476) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP10
Gene ID: 4319
Uniprot ID: MMP10_HUMAN
Immunogen Region: 237-476
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 237-476 of human MMP10 (NP_002416.1).
Immunogen Sequence: PLYNSFTELAQFRLSQDDVN GIQSLYGPPPASTEEPLVPT KSVPSGSEMPAKCDPALSFD AISTLRGEYLFFKDRYFWRR SHWNPEPEFHLISAFWPSLP SYLDAAYEVNSRDTVFIFKG NEFWAIRGNEVQAGYPRGIH TLGFPPTIRKIDAAVSDKEK KKTYFFAADKYWRFDENSQS MEQGFPRLIADDFPGVEPKV DAVLQAFGFFYFFSGSSQFE FDPNARMVTHILKSNSWLH
Function Can degrade fibronectin, gelatins of type I, III, IV, and V.weakly collagens III, IV, and V. Activates procollagenase.
Protein Name Stromelysin-2
Sl-2
Matrix Metalloproteinase-10
Mmp-10
Transin-2
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Stromelysin-2 antibody
Anti-Sl-2 antibody
Anti-Matrix Metalloproteinase-10 antibody
Anti-Mmp-10 antibody
Anti-Transin-2 antibody
Anti-MMP10 antibody
Anti-STMY2 antibody

Information sourced from Uniprot.org

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