Anti-MLXIPL antibody (17-175) (STJ29945)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MLXIPL (17-175) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MLXIPL |
| Gene ID | 51085 |
| Uniprot ID | MLXPL_HUMAN |
| Immunogen Region | 17-175 |
| Immunogen Sequence | VAPSPDSDSDTDSEDPSLRR SAGGLLRSQVIHSGHFMVSS PHSDSLPRRRDQEGSVGPSD FGPRSIDPTLTRLFECLSLA YSGKLVSPKWKNFKGLKLLC RDKIRLNNAIWRAWYIQYVK RRKSPVCGFVTPLQGPEADA HRKPEAVVLEGNYWKRRIE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 17-175 of human MLXIPL (NP_116569.1). |
Additional Info
| Tissue Specificity | Expressed in liver, heart, kidney, cerebellum and intestinal tissues. |
| Post Translational Modifications | Phosphorylation at Ser-556 by AMPK inactivates the DNA-binding activity. |
| Function | Binds DNA as a heterodimer with MLX/TCFL4 and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. Regulates transcription in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and gluconeogenesis, respectively. |
| Protein Name | Carbohydrate-Responsive Element-Binding ProteinChrebpClass D Basic Helix-Loop-Helix Protein 14Bhlhd14Mlx InteractorMlx-Interacting Protein-LikeWs Basic-Helix-Loop-Helix Leucine Zipper ProteinWs-BhlhWilliams-Beuren Syndrome Chromosomal Region 14 Protein |
| Database Links | Reactome: R-HSA-163358Reactome: R-HSA-163680Reactome: R-HSA-163765Reactome: R-HSA-163767 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Carbohydrate-Responsive Element-Binding Protein antibodyAnti-Chrebp antibodyAnti-Class D Basic Helix-Loop-Helix Protein 14 antibodyAnti-Bhlhd14 antibodyAnti-Mlx Interactor antibodyAnti-Mlx-Interacting Protein-Like antibodyAnti-Ws Basic-Helix-Loop-Helix Leucine Zipper Protein antibodyAnti-Ws-Bhlh antibodyAnti-Williams-Beuren Syndrome Chromosomal Region 14 Protein antibodyAnti-MLXIPL antibodyAnti-BHLHD14 antibodyAnti-MIO antibodyAnti-WBSCR14 antibody |
Information sourced from Uniprot.org