• Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1：1000
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of Hela cell. 1, AF-10 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV monoclonal antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MLLT10 antibody (211-260 aa) (STJ91504)

SKU:
STJ91504

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein AF-10 (211-260 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLLT10
Gene ID: 8028
Uniprot ID: AF10_HUMAN
Immunogen Region: 211-260 aa
Specificity: AF-10 Polyclonal Antibody detects endogenous levels of AF-10 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AF10 at the amino acid range 211-260
Function Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation.
Protein Name Protein Af-10
All1-Fused Gene From Chromosome 10 Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Af-10 antibody
Anti-All1-Fused Gene From Chromosome 10 Protein antibody
Anti-MLLT10 antibody
Anti-AF10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance