• Immunofluorescence analysis of NIH/3T3 cells using MLKL Rabbit polyclonal antibody (STJ119442) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using MLKL Rabbit polyclonal antibody (STJ119442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MLKL antibody (369-471) (STJ119442)

SKU:
STJ119442

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MLKL (369-471) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLKL
Gene ID: 197259
Uniprot ID: MLKL_HUMAN
Immunogen Region: 369-471
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 369-471 of human MLKL (NP_689862.1).
Immunogen Sequence: DRVKSTAYLSPQELEDVFYQ YDVKSEIYSFGIVLWEIATG DIPFQGCNSEKIRKLVAVKR QQEPLGEDCPSELREIIDEC RAHDPSVRPSVDEILKKLST FSK
Post Translational Modifications Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.
Protein Name Mixed Lineage Kinase Domain-Like Protein
Hmlkl
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-9686347
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus
Localizes To The Cytoplasm And Translocates To The Plasma Membrane On Necroptosis Induction
Localizes To The Nucleus In Response To Orthomyxoviruses Infection
Alternative Antibody Names Anti-Mixed Lineage Kinase Domain-Like Protein antibody
Anti-Hmlkl antibody
Anti-MLKL antibody

Information sourced from Uniprot.org

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