| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC |
| Reactivity: | Human/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MKRN1 (105-118) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 55kDa band observed in lysates of cell line THP-1 and in Human Testis lysates (calculated MW of 53.3kDa according to NP_038474.2). Recommended concentration: 0.1-0.5ug/ml. Prim |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MKRN1 |
| Gene ID: | 23608 |
| Uniprot ID: | MKRN1_HUMAN |
| Immunogen Region: | 105-118 |
| Accession Number: | NP_038474.2; NP_001138597.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1). |
| Immunogen Sequence: | RYEHSKPLKQEEAT |
| Post Translational Modifications | Auto-ubiquitinated.which leads to proteasomal degradation. |
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. |
| Protein Name | E3 Ubiquitin-Protein Ligase Makorin-1Ring Finger Protein 61Ring-Type E3 Ubiquitin Transferase Makorin-1 |
| Database Links | Reactome: R-HSA-198323Reactome: R-HSA-8948751Reactome: R-HSA-983168 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Makorin-1 antibodyAnti-Ring Finger Protein 61 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Makorin-1 antibodyAnti-MKRN1 antibodyAnti-RNF61 antibody |
Information sourced from Uniprot.org