• HEK293 lysate (10ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with STJ72940 (0. 5ug/ml) in right panel and with STJ72941 (0. 5ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.
  • STJ72940 and STJ72941 (1. 5ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with mouse anti-MK5/ PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).

Anti-MK5/MAPKAPK5 antibody (Internal) (STJ72940)

SKU:
STJ72940-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB-Trf/IP/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MK5/MAPKAPK5 (Internal) is suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-Recommended concentration, 4-8µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MAPKAPK5
Gene ID: 8550
Uniprot ID: MAPK5_HUMAN
Immunogen Region: Internal
Accession Number: NP_003659.2; NP_620777.1
Immunogen Sequence: STEALDNVLPSAQ
Post Translational Modifications Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4.which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated.
Function Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Protein Name Map Kinase-Activated Protein Kinase 5
Mapk-Activated Protein Kinase 5
Mapkap Kinase 5
Mapkap-K5
Mapkapk-5
Mk-5
Mk5
P38-Regulated/Activated Protein Kinase
Prak
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-5687128
Reactome: R-HSA-6804756
Cellular Localisation Cytoplasm
Nucleus
Translocates To The Cytoplasm Following Phosphorylation And Activation
Interaction With Erk3/Mapk6 Or Erk4/Mapk4 And Phosphorylation At Thr-182
Activates The Protein Kinase Activity
Followed By Translocation To The Cytoplasm
Phosphorylation By Pka/Prkaca At Ser-115 Also Induces Nuclear Export
Alternative Antibody Names Anti-Map Kinase-Activated Protein Kinase 5 antibody
Anti-Mapk-Activated Protein Kinase 5 antibody
Anti-Mapkap Kinase 5 antibody
Anti-Mapkap-K5 antibody
Anti-Mapkapk-5 antibody
Anti-Mk-5 antibody
Anti-Mk5 antibody
Anti-P38-Regulated/Activated Protein Kinase antibody
Anti-Prak antibody
Anti-MAPKAPK5 antibody
Anti-PRAK antibody

Information sourced from Uniprot.org

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