| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MITD1 (1-249) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MITD1 |
| Gene ID: |
129531 |
| Uniprot ID: |
MITD1_HUMAN |
| Immunogen Region: |
1-249 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human MITD1 (NP_620153.1). |
| Immunogen Sequence: |
MAKSGLRQDPQSTAAATVLK RAVELDSESRYPQALVCYQE GIDLLLQVLKGTKDNTKRCN LREKISKYMDRAENIKKYLD QEKEDGKYHKQIKIEENATG FSYESLFREYLNETVTEVWI EDPYIRHTHQLYNFLRFCEM LIKRPCKVKTIHLLTSLDEG IEQVQQSRGLQEIEESLRSH GVLLEVQYSSSIHDREIRFN NGWMIKIGRGLDYFKKPQSR FSLGYCDFDLRPCHETTVD |
| Function | Required for efficient abscission at the end of cytokinesis, together with components of the ESCRT-III complex. |
| Protein Name | Mit Domain-Containing Protein 1 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideMidbodyMembraneDuring CytokinesisRecruited To The Midbody Via Interaction With Chmp1aInteracts With Membranes Enriched In Phosphoinositides |
| Alternative Antibody Names | Anti-Mit Domain-Containing Protein 1 antibodyAnti-MITD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NDUFV2 knockout (KO) HeLa cells, using [KO Validated] NDUFV2 Rabbit polyclonal antibody (STJ11100812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89748/358225/STJ11100812_1__51306.1713122550.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NDUFV2 knockout (KO) HeLa cells, using [KO Validated] NDUFV2 Rabbit polyclonal antibody (STJ11100812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
