| Short Description : | Rabbit polyclonal MIOS (556-875) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MIOS |
| Gene ID: | 54468 |
| Uniprot ID: | MIOS_HUMAN |
| Immunogen Region: | 556-875 |
| Immunogen Sequence: | VVAMALSGYTDEKNSLWREM CSTLRLQLNNPYLCVMFAFL TSETGSYDGVLYENKVAVRD RVAFACKFLSDTQLNRYIEK LTNEMKEAGNLEGILLTGLT KDGVDLMESYVDRTGDVQTA SYCMLQGSPLDVLKDERVQY WIENYRNLLDAWRFWHKRAE FDIHRSKLDPSSKPLAQVFV SCNFCGKSISYSCSAVPHQG RGFSQYGVSGSPTKSKVTSC PGCRKPLPRCALCLINMGT |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 556-875 of human MIOS (NP_061878.3). |
| Function | As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex. Within the GATOR2 complex, MIOS is required to prevent autoubiquitination of WDR24, the catalytic subunit of the complex. The GATOR2 complex is required for brain myelination. |
| Protein Name | Gator2 Complex Protein MiosMissing Oocyte Meiosis Regulator Homolog |
| Database Links | Reactome: R-HSA-9639288 |
| Cellular Localisation | Lysosome Membrane |
| Alternative Antibody Names | Anti-Gator2 Complex Protein Mios antibodyAnti-Missing Oocyte Meiosis Regulator Homolog antibodyAnti-MIOS antibody |
Information sourced from Uniprot.org
