| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MICU1 |
| Gene ID: |
10367 |
| Uniprot ID: |
MICU1_HUMAN |
| Immunogen Region: |
105-255 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 105-255 of human MICU1 (NP_001182447.1). |
| Immunogen Sequence: |
GFRDRKVMEYENRIRAYSTP DKIFRYFATLKVISEPGEAE VFMTPEDFVRSITPNEKQPE HLGLDQYIIKRFDGKKISQE REKFADEGSIFYTLGECGLI SFSDYIFLTTVLSTPQRNFE IAFKMFDLNGDGEVDMEEFE QVQSIIRSQTS |
| Tissue Specificity | Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells. |
| Post Translational Modifications | Phosphorylation at Ser-122 by AKT1 impairs its maturation and stability. Asymmetric dimethylation at Arg-455 by PRMT1 decreases the calcium sensitivity of MICU1 by promoting interaction with UCP2. Degraded by YME1L1 when not complexed as homodimer or heterodimer. Not degraded when complexed as homodimer or heterodimer.the presence of the interchain disulfide bond protecting MICU1 from degradation by YME1L1. |
| Function | Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains. MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake. At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel. Also required to protect against manganese toxicity by preventing manganese uptake by MCU: mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion. Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360. Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG. Isoform 6: Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle. Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations. This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise. |
| Protein Name | Calcium Uptake Protein 1 - MitochondrialAtopy-Related Autoantigen CalcAra CalcCalcium-Binding Atopy-Related Autoantigen 1Allergen Hom S 4 |
| Database Links | Reactome: R-HSA-8949215Reactome: R-HSA-8949664 |
| Cellular Localisation | Mitochondrion Intermembrane SpaceMitochondrion Inner MembraneRecruited To The Mitochondrial Inner Membrane By Emre/Smdt1Also Localizes To Mitochondrial Cristae JunctionsIsoform 6: Mitochondrion Intermembrane Space |
| Alternative Antibody Names | Anti-Calcium Uptake Protein 1 - Mitochondrial antibodyAnti-Atopy-Related Autoantigen Calc antibodyAnti-Ara Calc antibodyAnti-Calcium-Binding Atopy-Related Autoantigen 1 antibodyAnti-Allergen Hom S 4 antibodyAnti-MICU1 antibodyAnti-CALC antibodyAnti-CBARA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
