Anti-MFN1 antibody (163-213 aa) (STJ193472)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human MFN1 at the amino acid range 163-213
General Information
| Short Description | Rabbit polyclonal anti-Mitofusin-1 (163-213 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MFN1 |
| Gene ID | 55669 |
| Uniprot ID | MFN1_HUMAN |
| Immunogen | Synthesized peptide derived from the human MFN1 at the amino acid range 163-213 |
| Immunogen Region | 163-213 aa |
| Specificity | This antibody detects endogenous levels of MFN1 at Human/Mouse/Rat |
Additional Info
| Post Translational Modifications | Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion. Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN. |
| Function | Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity. |
| Protein Name | Mitofusin-1Fzo HomologTransmembrane Gtpase Mfn1 |
| Database Links | Reactome: R-HSA-5205685Reactome: R-HSA-9013419Reactome: R-HSA-983231 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane ProteinIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Mitofusin-1 antibodyAnti-Fzo Homolog antibodyAnti-Transmembrane Gtpase Mfn1 antibodyAnti-MFN1 antibody |
Information sourced from Uniprot.org