| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MDH1 |
| Gene ID: | 4190 |
| Uniprot ID: | MDHC_HUMAN |
| Immunogen Region: | 1-334 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MDH1 (NP_005908.1). |
| Immunogen Sequence: | MSEPIRVLVTGAAGQIAYSL LYSIGNGSVFGKDQPIILVL LDITPMMGVLDGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSMPRREGMERKD LLKANVKIFKSQGAALDKYA KKSVKVIVVGNPANTNCLTA SKSAPSIPKENFSCLTRLDH NRAKAQIALKLGVTANDVKN VIIWGNHSSTQYPDVNHAKV KLQGKEVGVYEALKDDSWLK GEFVTTVQQRGAAVIKARK |
| Post Translational Modifications | ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation. |
| Function | Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS). |
| Protein Name | Malate Dehydrogenase - CytoplasmicAromatic Alpha-Keto Acid ReductaseKarCytosolic Malate Dehydrogenase |
| Database Links | Reactome: R-HSA-9856872 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Malate Dehydrogenase - Cytoplasmic antibodyAnti-Aromatic Alpha-Keto Acid Reductase antibodyAnti-Kar antibodyAnti-Cytosolic Malate Dehydrogenase antibodyAnti-MDH1 antibodyAnti-MDHA antibody |
Information sourced from Uniprot.org