• STJ70370 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • STJ70370 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). NA NA NA IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-MDA5/IFIH1 antibody (N-Term) (STJ70370)

SKU:
STJ70370-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MDA5/IFIH1 (N-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-10ug/ml
FC-Flow cytometric analysis of K562 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: IFIH1
Gene ID: 64135
Uniprot ID: IFIH1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_071451.2
Immunogen Sequence: SNGYSTDENFRYL
Post Translational Modifications Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta. Ubiquitinated by RNF125, leading to its degradation by the proteasome. USP17/UPS17L2-dependent deubiquitination positively regulates the receptor. Ubiquitinated by TRIM25 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. Ubiquitinated by TRIM40 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. Ubiquitinated by TRIM65 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. ISGylated by ISG15. ISGylation increases upon infection with dengue (DENV) or Zika (ZIKV) viruses. ISGylation at Lys-23 and Lys-43 is dependent of dephosphorylation at Ser-88, regulates mitochondrial translocation and oligomerization. Essential for IFIH1/MDA5-mediated cytokine responses and restriction of virus replication. Phosphorylated at Ser-88. Dephosphorylated by phsophatases PP1.dephosphorylation precedes and is required for ISGylation. During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. (Microbial infection) Cleaved and inactivated by the protease 2A of coxsackievirus B3, poliovirus and enterovirus 71 allowing the virus to disrupt the host type I interferon production.
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs).IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2. Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
Protein Name Interferon-Induced Helicase C Domain-Containing Protein 1
Clinically Amyopathic Dermatomyositis Autoantigen 140 Kda
Cadm-140 Autoantigen
Helicase With 2 Card Domains
Helicard
Interferon-Induced With Helicase C Domain Protein 1
Melanoma Differentiation-Associated Protein 5
Mda-5
Murabutide Down-Regulated Protein
Rig-I-Like Receptor 2
Rlr-2
Rna Helicase-Dead Box Protein 116
Database Links Reactome: R-HSA-168928
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-933542
Reactome: R-HSA-933543
Reactome: R-HSA-936440
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Upon Viral Rna Stimulation And Isgylation
Translocates From Cytosol To Mitochondrion
May Be Found In The Nucleus
During Apoptosis
Alternative Antibody Names Anti-Interferon-Induced Helicase C Domain-Containing Protein 1 antibody
Anti-Clinically Amyopathic Dermatomyositis Autoantigen 140 Kda antibody
Anti-Cadm-140 Autoantigen antibody
Anti-Helicase With 2 Card Domains antibody
Anti-Helicard antibody
Anti-Interferon-Induced With Helicase C Domain Protein 1 antibody
Anti-Melanoma Differentiation-Associated Protein 5 antibody
Anti-Mda-5 antibody
Anti-Murabutide Down-Regulated Protein antibody
Anti-Rig-I-Like Receptor 2 antibody
Anti-Rlr-2 antibody
Anti-Rna Helicase-Dead Box Protein 116 antibody
Anti-IFIH1 antibody
Anti-MDA5 antibody
Anti-RH116 antibody

Information sourced from Uniprot.org

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